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Abstract:

In this work, the total carbonic anhydrase (CA) enzymes were obtained from bighead carp (Hypophthalmichthys nobilis) gill tissue with a purification fold of 239.62, specific activity of 26128.18 EU mg^-1 and a yield of 44.04 % using Sepharose affinity column chromatography. For recording the CA purity, gel electrophoresis was performed in this part. The molecular weight (MW) of total CA enzyme was found 31 kDa. Additionally, the inhibitory effects of diverse pesticide compounds (carbaryl, carbofuran, permethrin, dimethoate, cypermethrin and λ-cyhalothrin) and heavy metals (Cu²⁺, Fe²⁺, Pb²⁺, Co²⁺) on CA bighead carp gill tissue CA enzyme activities were investigated and also the results calculated, and then plotted. The some pesticide compounds had IC50 amounts in the range of 0.36 - 253.35 μM. Also, the metal ions used had IC50 values ranging from 7.84 to 95.28 mm. As a result of these studies, a stable pH is set as pH: 8.0. CA inhibitors are target molecules in drug design studies.