Few climatic parameters available for the stratigraphic interval between Aquitanian – Pontian from Romania, have been obtained after analysis of the palynological associations using the Coexistence Approach. The results obtained for MAT (mean annual temperature), MAP (mean annual precipitations), WMT (mean temperature of the warmest month), CMT (mean temperature of the coldest month), have been synthesised and palaeoclimatic diagrams have been plotted, revealing visible climatic fluctuation for the analysed interval. Palaeoclimatic curve was calculated for Dacian Basin, covering the southern and eastern part of Romania and Transylvanian Basin (Central-Western area of the country). MAT with high value have been observed for the Burdigalian (approximately 18.4°C), then a general trend of cooling was observed. The calculated values of MAP from the Miocene reach from 957–1353 mm. Beginning with the Late Miocene, a slight cooling and some drying is recorded in Dacian Basin due to a regional palaeogeographic reorganizations and tectonic processes. Our study provides a new insight into palaeoclimatic evolution from Romania, based on palynological data.