The article reports the chemical characteristic of acid mining drainage waters as well as the results of leaching experiments conducted with and Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans at the same conditions in solution. The experiments were realized realized using tailing impoundment sediments and ore minerals from the Sb-(Au-) base metal deposit Pezinok (Malé Karpaty Mts., Western Carpathians, Slovakia). The research study shows the oxidation sequence and the leaching progression on surface of the following ore minerals: löllingite, arsenopyrite, stibnite, native Sb, gudmundite, berthierite, pyrite, sphalerite and chalcopyrite. The differences between chemical and biological-chemical leaching activity of various ore minerals on the polished sections surface is discussed. The extent and the kinetics of the biological-chemical leaching of ore minerals is significantly higher than the chemical leaching of ore minerals without bacteria.